Practical Training

CONTENT

Why this Training
What we have proposed to do
Person to be contacted
Our Available Facilities
Laboratories

1. Computer laboratories
2. Protein purification facility
3. The molecular biology
4. The microbiology facility
5. Acute tissue culture facility

Facilitation
Entrepreneurship
Corporate Education
On going Programme

Difference Courses

1. Clinical Biochemistry
2. Molecular Biology and Genetic Engineering
3. Medical and Industrial Microbiology
4. Industrial Microbiology
5. Immunology

Summer Projects for Students

1. B.Pharm Students
2. Bio-tech Students

Practical Course content
Day-to-day schedule of the course for 15 days training programme

SHORT TERM TRAINING AND SUMMER PROJECTS

Why this Training

The Government of India in its Vision 2020, has identified Biotechnology as one of the growth engines for the Country and has undertaken rapid strides to develop the sector. Govt invites the private players to promote the sector but still the country lacks appropriate number of skilled hands in the field of Biotechnology & Pharmacy. Unfortunately, Varanasi has not emerged as the destination of first choice for corporate and multinationals. Although, NOIDA has attracted some Industrial houses, but still it is lagging behind as compared to the other metro cities, Bangalore, Pune and Hyderabad.

As we all know that vision with action is a powerful empowerment, therefore Prof SN Tripathi Memorial Foundation (829/92-93) established in 1992 on the initiative of Late Professor K N Udupa to fulfil the incomplete goals of Late Professor S. N. Tripathi, Ex Dean Faculty of Ayurveda, Banaras Hindu University. Prof Tripathi had a firm belief that “Ayurvda is a Science and it can only be proved at the molecular level”. He was of opinion that only good scientists and most advance laboratories can help in the proper development of Ayurveda.

As charity begins at home, late Prof S.N. Tripathi motivated his eldest son Dr Yamini Bhusan Tripathi, PhD in Biochemistry, now Professor of Medicinal Chemistry, Faculty of Ayurveda to do research in the field of Ayurveda as early as in 1979. Later on he motivated his youngest son Vachaspati Tripathi, M Pharm from Institute of Technology, BHU to establish a company for manufacturing only Ayurvedic drugs in 1983. Now this is the only GMP certified company named as Surya Pharmaceuticals in this area and has pride to export its medicines, around the world as per FDA norms of USA. Prof. Tripathi further motivated his another son Dr. Bamdeo Tripathi to study the effect of season an Health. He is now chief Geophysist in ONGC.

Now, although science and technology has progressed so much in the country, but it is really not being utilized for the development of Ayurveda, which can be clearly seen by the AICTE website, as there is no approved course for the development of herbal industry.

What we have proposed to do

Therefore, with an aim to produce an army of well trained scientists, specialized in the field of herbal industry, we have decided to train the MSc students and B Tech students with a holistic approach of chemistry, pharmacy, biotechnology, medical, biology, computer and agriculture Sciences. There is no such course, available with such integration of various subjects.

Besides, we are giving short-term training to the students of M Sc and B tech. We are also giving an opportunity to take up 6 months project by these students as the part of their semester.

We have set up a Cell for guiding the entrepreneurs and for filing applications related to the Intellectual Property Rights (IPR). We are in the process of setting up of a legal cell to solve the patent disputes.

We have developed a small Incubators under this center to provide common facilities to those Biotech companies and herbal industries which do not have their own standardization laboratory and R & D section.

PhD students from different institutions may also utilize this facility and work here to achieve their objectives, which is not possible in their parent Institutions.

We have applied for setting up an specialized animal house facilities for laboratory experimentation, approved by animal welfare committee. We have developed collaboration with various Universities and leading laboratories of Science and technology across the world. The resource persons will be invited from these institutions Group discussion and guest lectures will be organized from time to time. For advance techniques and instrumentations, academic tours will also be organized.

The focus will be on diagnostics, therapeutics, pharmacogenomics, bioinformatics, agriculture, industrial, marine forest and environment biotechnologies and aqua culture, drug development standardization and newer formulations. It will cover education in alternative medicines, Ayurveda to modern doctors.

Person to be contacted

(Dr Pratibha Tripathi)
Chief Coordinator
Research and Development Centre
Prof. S.N. Tripathi Memorial Foundation
1, Gandhi Nagar, Naria, Varanasi-221005
Tel : 0452-2366577, Cell : 9415694450
E-mail : pratibhaybt30@yahoo.com

Our Available Facilities

A hassle-free environment, compactable class room with all teaching aids with 24-hour high speed internet connectivity, uninterrupted power supply, round the clock security, good maintenance of all equipment. It is an ideal place for any motivated knowledge seeker. Staff and faculty (in-house and visiting) are all very accessible for help and advice. The friendly, congenial atmosphere makes working a pleasure. The library, classrooms, computer labs and wet labs are detailed in sections the following.

LABORATORIES

1. Computer laboratories: The computer lab houses computers capable of handling advanced bioinformatics software and molecular graphics, for in silico biology.
2. Protein purification facility: The protein purification facility includes all infrastructure and equipment required for routine isolation and downstream processing of proteins of interest from crude samples: Chromatography can be carried out at ambient temp. and purity and activity may be assayed by standard analytical techniques.
3. The molecular biology lab has facilities for protein analysis genetic analysis and comparative studies. The equipment and reagents required for study of apoptosis, isolation of DNA and RNA are all available under one roof.
4. The microbiology facility includes sterile hoods, autoclaves, microscopes, incubators and shakers for growing and screening of microbes.
5. Acute tissue culture facility for eukaryotic cells is available, and enables the student to work with macrophages, lymphocytes, thymocytes and splennocytes. A clean environment and following of stringent rules minimizes the problems associated with contamination.

Facilitation

Besides the physical infrastructure, we take care of Placements, Entrepreneurial counseling and Mentor the alumni by maintaining active links with them.

Entrepreneurship

We promote entrepreneurship through an entrepreneurship club. In addition there are several programs for and by students in this field. In fact, we give small project through different industrial houses, who wish to take our help in developing their industries.

Corporate Education

We do plan in future to educate the corporate executive and scientists, by offering certain modules in different specialization of industrial sector.
Genes to Drugs: In-Silico Drug Discovery
A Lab-Course in Biotechnology
Patent Agent Training
Bio-Agriculture & Rural technology
Workshop on Pharmaceutical Education & Research Workshop on Bio-diesel
Global Regulatory & Quality Standards in Clinical Trials
Role of Statistics in Clinical Trials
Workshop on Molecule Drug Design
Introduction to Bioinformatics and Biotechnology
Information Technology in Healthcare
Herbal Industries development cultivation & collection of Medicinal plants Research paper & research project writing.

On going Programme

Certificate course in Herbal Drug Industry
UG Diploma course in Herbal Drug Industry (Proposed)
Short term Training to M Sc and B Tech Students (Summer & winter Training)
Project work for M Sc and B Tech Students
Incubator for Ph D students
Incubator for Industrial houses for selected experiments and projects
Contract research

Difference Courses

1. Clinical Biochemistry
   1. Different methods of blood collection
   2. Storage, Transportation and Processing of Clinical specimens
   3. Separation of serum & plasma from blood
   4. Qualitative and quantitative analysis of urine samples
   5. Assessment of Body/ Mass Index (BMI)
   6. Diabetes mellitus : Estimation of glucose from blood and urine
   7. Anemia: Level of iron and hemoglobin in blood
   8. Demonstration for the assay of Glycosylated Hb and Insulin in blood samples
   9. Cardiac risk assessment by using kits
      • Estimation of fibrinogen
      • Estimation of Lipid profile – Estimation of cholesterol, HDL, LDL and triglycerides
      • Estimation of Lactate dehydrogenase
           Clotting time
   10. Liver function tests
       • Determination of SGOT and SGPT activity
       • Estimation of bilirubin
       • Determination of Albumin/Globulin ratio in serum
            Estimation of Alkaline phosphatase
   11. Renal function tests
       • Estimation of serum creatinine
       • Analysis of serum urea
       • Estimation of uric acid
            Estimation of serum proteins
2. Molecular Biology and Genetic Engineering
   
   1. Orientation and instrumentation
   2. Calculations and Chemical preparation
   3. Isolation of DNA
   4. Isolation of RNA
   5. Agarose Gel Electrophoresis for DNA and RNA
   6. Poly accrylamide gel electrophoresis of Proteins (Native PAGE and SDS PAGE)
3. Medical and Industrial Microbiology
   
   1. Different methods of blood collection from animals and Human
   2. Storage, Transportation and Processing of Clinical specimens
   3. Separation of serum & plasma from blood
   4. Blood smear preparation, Staining & visualization of blood cells, Blood counts: TLC, DLC
   5. Measurement of RA factor, Pregnancy tests, CRP test
   6. Different Staining Methods
      • Gram stain
      • Capsular
      • Acid-fast
      • Endospore
      • Albert’s
   7. Erythrocyte Sedimentation Rate (ESR)
   8. Packed Cell Volume (PCV)
   9. Estimation of Blood hemoglobin using Haemometer
   10. Blood clotting related parameters
   11. Separation of serum by native Poly Acrylamide Gel Electrophoresis Agarose gel Electrophoresis
4. Industrial Microbiology
   1. Media preparation and Sterilization
   2. Isolation & screening for industrially important bacteria
   3. Assessment of Microbial load on herbal drugs Colony counting
   4. Microbial Culture and growth curve of microorganism
   5. Bacterial Motility
      • Microscopic
      • Macroscopic
   6. Immobilization of Cells
   7. Testing and efficacy of antimicrobial drugs
   8. Quality analysis of disinfectants
      Analysis of water
   9. Detection & determination of alcohol by chromic acid method
   10. Thin Layer chromatography
   11. Chromatography of fat and oils
   12. Analysis of water
   13. Quality analysis of milk
5. Immunology
   1. Different methods of blood collection from animals
   2. Separation of serum & plasma from blood
   3. Clinical specimen collection, Storage, Transportation and Processing
   4. Media preparation and Sterilization
   5. Separation of serum by native Poly Acrylamide Gel Electrophoresis Antigen- Antibody Titer
   6. Measurement of serum albumin
   7. Separation of serum aminoacids by paper chromatography
   8. Enzymology and biochemical analysis for
      • Catalase
      • Urease
   9. Technique of Gel filtration chromatography
   10. Agarose Gel electrophoresis of Lipoproteins Immunodiffusion technique
       Antigen- Antibody titer
       Allergy test.
   11. Direct & indirect Antiglobulin Test (Coomb’s test)

Projects

1. For students of M Sc in Biochemistry, life sciences, genetics, Experimental medicine, biochemistry, pharmacology and Pharmacy

Tenure 3 months
Price -15,000/=

A. Bio-tech Students
Effect of Drugs on lipid lowering and antioxidant property
Effect of Drugs on asthma and antioxidant property
Effect of Drugs on diabetes and antioxidant property
Effect of Drugs on wound healing and antioxidant property
Effect of Drugs on stress induced changes and antioxidant property
Effect of Drugs on learning and memory in rats
Effect of Drugs on inflammation antioxidant property
Effect of Drugs on inflammation antioxidant property

Curriculum

Handling of experiments
Ethical issues related to animal experiments
Introduction to various analytical techniques
Principles of spectrophotometry
Free radicals and antioxidants
Free radicals and diseases
Project writing skills
Principles of experimental medicine
Toxicity and safety in drug action
Adverse drug reactions
Standardization of herbal drugs

B. Pharm students

Projects

Phyto-chemical analysis of herbal drug
Pharmacognostical analysis of herbal drugs
Chemical Analysis of different pharmaceuticals samples
Analysis of antimicrobial drugs and pharmaceuticals
Safety and toxicity of herbal drugs
Development of new formulations
Development of new herbal ointments
Study of Drug metabolizing activity
Chemotaxonomy and genotaxonomy of plants
Curriculum
Analysis of herbal drug
Chemical Standardization of herbal drugs
Working in Tablet section and its standardization
Working in Syrup section and its standardization
Working in powder section and its standardization
Quality control measures in a pharmaceutical company
Introduction to various analytical techniques
Principles of spectrophotometry

Course content (please specify schedule of lectures and practical)

Practical

1. Isolation and characterization of (1) small molecules from plants,
2. Thin layer chromatography
3. Column chromatography
4. Melting point determination
5. Determination of moisture content
6. Determination of Ash content
7. Determination of microbial load in the herbal samples
8. Determination of quality of syrup, powder and tablets on standard parameters
9. Determination of phytochemical contents
10. Determination of alkaloid content
11. Microscopy of plant samples
12. Isolation and characterization of protein (urease)
13. Isolation and characterization of enzyme Superoxide dismutase from RBC (activity staining)
14. Isolation and characterization of DNA from animal tissues
15. Isolation and characterization of DNA from bacterial culture
16. Culture of bacteria and growth curve.
17. Culture of peritoneal macrophages and its viability by trypan blue exclusion method.
18. Radial diffusion on agarose plate.
19. Antigen –anti body titer determination
20. Determination of blood group
21. Biochemical estimation of blood glucose, cholesterol
22. Determination of blood HB,
23. Identification of blood cells and their counting
24. Microbial analysis of herbal producer.
25. Training to manufacture Surya dosage form
26. Training to manufacture Tablet dosage form
27. Training to manufacture Capsule dosage form
28. Training to manufacture Powder dosage form
29. Training to manufacture gelatin dosage form
30. Training other pharmaceutical fundamental
31. Extra use in GMP & GLP

( Note : Depending on the type of industry, the participants will be classified in to different groups and specific experiments will be taught to them)